|
KMID : 0525720090140040192
|
|
Journal of Chitin and Chitosan
2009 Volume.14 No. 4 p.192 ~ p.196
|
|
|
Antioxidative Effects of Sulfated Chitooligosaccharides on Oxidative Injury
|
|
Lee Seung-Jae
Kim Eun-Kyung
Hwang Jin-Woo
Oh Hyun-Jung
Park Pyo-Jam
Kim Chan-Gil
|
|
|
Abstract
|
|
|
Protective effects of sulfated chitosan and chitooligosaccharides were evaluated by employing a fluorescence activated cell sorter (FACS) on hydrogen peroxide and plasmid DNA strand breaks assay. We prepared sulfated chitosan (SC) and sulfated chitooligosaccharides (SCOS I, SCOS II and SCOS III) from 90% deacetylated chitosan and three kinds of
chitooligosaccharides (COS I; 5,000-10,000 Da, COS II; 1,000-5,000 and COS III; below the 1,000). The SCOS III showed the
highest protective activities in liver cells, with cells apoptosis percentage values of 22.54% at 1.0 mg/mL. In addition, the SCOS III exhibited a protective effect on H2O2-induced DNA damage. These results indicate that SCOS III possesses potent protective effects on liver cells and DNA against H2O2-induced oxidative damage.
|
|
|
KEYWORD
|
|
|
apoptosis, DNA protective effect, sulfated chitosan, sulfated chitooligosaccharides
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|